We further provide an immunohistochemistry procedure to assess the colocalization of isolated astrocytes with synaptic markers throughout the z-plane. Astrocytes are imaged using confocal microscopy, and reconstructed in three dimensions to obtain detailed morphometric data. This tool and approach allows for visualization of a single isolated astrocyte in its entirety, including fine peripheral processes. Herein, we describe a method utilizing a commercially available and validated, genetically-encoded membrane-associated fluorescent marker of astrocytes, AAV-GfaABC1D-Lck-GFP. The majority of studies examining the morphology of astrocytes have employed immunoassays for markers such as GFAP, which are insufficient to encapsulate the considerable structural complexity of these cells. Accordingly, it is of merit to identify structural and functional properties of astrocytes in both health and disease. Astrocytes play numerous vital roles in the central nervous system.
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